Imaging Core Facility

Usage discount unstaffed facility hours, including holidays and weekends

Standard dehydration series including increasing concentrations of Ethanol, Xylene/Ethanol mixture, and pure xylenes.

 Imaging or tiling of one slide at 40x magnification.  Up to four standard colors depending on combination (dapi, gfp, cfp, yfp, rhodamine, texas red, CY5)

Imaging Facility Transcriptomics Sample Preparation and Library Construction - Includes RNAse free sample processing, sectioning, staining, DSP instrument programming and setup (including calibration file upload), Library Readout prep, transfer to sequencing facility, post-sequencing file translation and upload to DSP instrument, and sample normalization for analysis.  Price is per 96-well plate regardless of number of wells filled.  Price does not include sequencing charges or analysis beyond data normalization.

Imaging Facility Transcriptomics Sample Preparation and Library Construction - Includes RNAse free sample processing, sectioning, hybridization, morphology marker staining (optimized reagents), DSP instrument programming and setup (including calibration file upload), Library Readout prep, and transfer to sequencing facility.  Price is per 96-well plate regardless of number of wells filled.  Price does not include sequencing charges or analysis beyond data normalization.

Removing and reattaching sample in a different orientation due to ROI specificity and/or knife angle limitations.

20, 25 or 50um intact paraffin curls for Visium single cell processing procedure (2 total)

Usage discount unstaffed facility hours, including holidays and weekends

Removing and reattaching sample in a different orientation due to ROI specificity and/or knife angle limitations.

Standard dehydration series including increasing concentrations of Ethanol, Xylene/Ethanol mixture, and pure xylenes.

Imaging Facility Transcriptomics Sample Preparation and Library Construction - Includes RNAse free sample processing, sectioning, staining, DSP instrument programming and setup (including calibration file upload), Library Readout prep, transfer to sequencing facility, post-sequencing file translation and upload to DSP instrument, and sample normalization for analysis.  Price is per 96-well plate regardless of number of wells filled.  Price does not include sequencing charges or analysis beyond data normalization.

Imaging Facility Transcriptomics Sample Preparation and Library Construction - Includes RNAse free sample processing, sectioning, hybridization, morphology marker staining (optimized reagents), DSP instrument programming and setup (including calibration file upload), Library Readout prep, and transfer to sequencing facility.  Price is per 96-well plate regardless of number of wells filled.  Price does not include sequencing charges or analysis beyond data normalization.

20, 25 or 50um intact paraffin curls for Visium single cell processing procedure (2 total)

 Imaging or tiling of one slide at 40x magnification.  Up to four standard colors depending on combination (dapi, gfp, cfp, yfp, rhodamine, texas red, CY5)